this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

Successful anther/microspore culture largely depends on the use of microspores at appropriate developmental stages at the time of culture, which can be specific for each plant species and genotype. This study was carried out to determine the correlation between morphological characteristics, namely floral bud (FB) length and width and anther length, and specific microspore developmental stages ...

Pollen plants from anther culture in rice breeding allows selection of fine strains in early generations. Exploitation of anther culture technique in research is limited due to very low regeneration frequency of indica cultivars and the transfer of anther culture traits from japonica to indica varieties is important in improving these rice varieties. In this study the optimization of media requ...

Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from tran...

Abstract Plant tissue culture is effective for plant propagation or biotechnology purposes, one of which anther culture. Anther pepper difficult because callus growth, regeneration organs, and low effectiveness. Pre-treatment such as cold temperatures can increase the induction growth embryogenesis in cultures. This study aimed to determine effect pre-treatment period on formation local Aceh pl...

the objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. two wheat cultivars of grebe and houtman were reciprocally crossed with two synthetic genotypes of do1 and pol and then a complete set of the parents, f1, reciprocal f1 (rf1), f2 and back-cross generations (bc1 and bc2) of each cross were used for anthe...

The influence of cultivar, donor plant and culture procedure on the efficiency of androgenesis was studied in carrot anther culture. Experiments were carried out on five carrot cultivars: CxC 9900 F1, Lucky B F1, HCM, Beta III and Perfekcja, which were chosen because of their high carotene contents. Two procedures of anther culture were compared: (1) incubation in darkness for two weeks, follow...